肿瘤的发生发展是一个复杂的过程，涉及一系列遗传和表观遗传的改变。这些改变使得细胞能够摆脱正常生长调控信号（如外部环境和内部信号）的束缚，获得自主增殖的能力。其中，锚定非依赖性生长（Anchorage-independent growth）是细胞恶性转化的重要标志之一，而软琼脂集落形成实验（Soft Agar Colony Formation Assay）被认为是检测细胞恶性转化的金标准。

软琼脂集落形成实验通过在半固体培养基中培养细胞，观察其是否能够在无锚定条件下形成集落，从而判断细胞的恶性转化能力。然而，这种方法通常需要3-4周才能获得结果，严重拖慢研究进度，并且由于依赖人工显微镜计数集落，难以统一判定导致结果偏差。最重要的是传统软琼脂实验无法回收活细胞，限制了后续研究的开展。

Cellbiolabs新一代CytoSelectTM检测系统结合荧光定量技术和改良软琼脂配方，全面优化了实验流程：

CytoSelectTM细胞转化检测试剂盒通过高灵敏度的荧光检测法定量细胞集落，实验周期从3-4周缩短至1周内，显著提高了研究效率，同时避免了人工计数的主观误差，尤其适合高通量样本检测。对于蛋白质/DNA芯片分析或癌症疫苗开发等应用领域而言，使用专用改良软琼脂培养基可以轻松回收活化的转化细胞供进一步培养和测试使用。

Fig 1 细胞活力检测：按照实验方案回收HeLa和293细胞培养6天，用台盼蓝法测定细胞活力。

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产品部分已发表文献：

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3. El Baba, R. et al. (2023). EZH2-Myc driven glioblastoma elicited by cytomegalovirus infection ofhuman astrocytes. Oncogene. doi: 10.1038/s41388-023-02709-3.

4. Kantisin, S. et al. (2022). In utero arsenic exposure increases DNA damage and gene expressionchanges in umbilical cord mesenchymal stem cells (UC-MSCs) from newborns as well as in UCMSC differentiated hepatocytes. Toxicol Rep. doi: 10.1016/j.toxrep.2022.09.002.

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参考文献

1. El Baba, R. et al. (2023). Polyploidy, EZH2 upregulation, and transformation in cytomegalovirus infected human ovarian epithelial cells. Oncogene. doi: 10.1038/s41388-023-02813-4.

2. Hiroki, H. et al. (2023). Targeting Poly(ADP)ribose polymerase in BCR/ABL1-positive cells. Sci Rep. 13(1):7588. doi: 10.1038/s41598-023-33852-2.

3. El Baba, R. et al. (2023). EZH2-Myc driven glioblastoma elicited by cytomegalovirus infection of human astrocytes. Oncogene. doi: 10.1038/s41388-023-02709-3.

4. Kantisin, S. et al. (2022). In utero arsenic exposure increases DNA damage and gene expression changes in umbilical cord mesenchymal stem cells (UC-MSCs) from newborns as well as in UC-MSC differentiated hepatocytes. Toxicol Rep. doi: 10.1016/j.toxrep.2022.09.002.

5. Nehme, Z. et al. (2022). Polyploid giant cancer cells, EZH2 and Myc upregulation in mammary epithelial cells infected with high-risk human cytomegalovirus. EBioMedicine. 80:104056. doi: 10.1016/j.ebiom.2022.104056.

Cell Biolabs公司坐落于美国加利福尼亚州圣地亚哥市，一直致力于开发生命科学研究领域的技术和工具，并将所开发的创新性技术成果商业化。Cell Biolabs孜孜不倦的完善产品，以期使细胞功能和疾病机制研究达到新高度。Cell Biolabs公司的产品独具特色并且处于行业前沿水平，全球众多大学、政府研究机构、生物、制药企业的科研实验室均在使用。

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